Suthakaran Pichaimuthu
Micro Therapeutic Research Labs, India
Title: High level intracellular expression of recombinant streptokinase as an inclusion body in E.coli
Biography
Biography: Suthakaran Pichaimuthu
Abstract
Streptokinase, a 414 amino acid polypeptidewithmolecularweightof 47kDa, is a potent activator of the fibrinolytic system in humans.High level expression of recombinant proteinin E.coli frequently results in accumulation of protein as in soluble aggregates known as inclusion bodies and they do offer several advantages. Expression as inclusion bodies is useful to obtain large amount of the protein, provided refolding is not difficult and recovery of the active protein is high. This is particularly true with the proteins not having disulfide bonds. Since Streptokinase does not have disulfide bonds, the focus is to obtain higher quantity of stable protein as inclusion bodies. In the present study, an attempt was made to investigate the effect of temperature and post induction time on stable (non-degradable) expression of recombinant streptokinase of highercontentas inclusion body in Escherichiacoli.