Vikas Bajpai
CSIR- Central Drug Research Institute, India
Title: Ultra performance liquid chromatography mass spectrometric analysis for rapid quantitation of isoquinoline alkaloids in Thalictrumreniforme
Biography
Biography: Vikas Bajpai
Abstract
Thalictrum (Ranunculaceae) is very widely distributed genus of medicinal plants in India and has been reported to have many therapeutic properties like diuretic, stomachic, antiseptic, aperient etc. Approximately 290 alkaloids were reported from the 80 species of the genus Thalictrum. Alkaloids from Thalictrum are reported to exhibit various pharmacological activities like antiamebic, antimicrobial, antitumour and antiviral. Protoberberine and aporphine alkaloids are reported the major components in this plant In present study, we present simultaneous quantification of some alkaloids in Thalictrumreniformeusing ultra performance liquid chromatography coupled to hybrid triple quadrupole/linear ion trap mass spectrometry (UPLC-QqQLIT-MS) operated in the multiple reactions monitoring acquisition. The separation was achieved on an ACQUITY UPLC CSHâ„¢ C18 column using a gradient mobile phase at flow rate of 0.3 mL/min. Stock solutions of standards were prepared in methanol within the ranges from 0.5 to 1500 ng/mL.The calibration curves were constructed by plotting the peak areas versus the concentrations of each analyte. Five PBAs were quantified in which Berberine, Jatrorrhizine and Palmatine were detected in significant amount.